DNA polymerase beta null mouse embryonic fibroblasts harbor a homozygous null mutation in DNA polymerase iota
نویسندگان
چکیده
منابع مشابه
DNA Polymerase Mediates a Back-up Base Excision Repair Activity in Extracts of Mouse Embryonic Fibroblasts*
Mammalian DNA polymerase (pol) is a member of the X-family of DNA polymerases and has striking enzymatic and structural similarities to mammalian DNA pol . Because pol provides two important enzymatic activities for base excision repair (BER), we examined whether pol might also contribute to BER. We used extracts from mouse embryonic fibroblasts representing wild-type and null genotypes for pol...
متن کاملDetection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism...
متن کاملAP endonuclease knockdown enhances methyl methanesulfonate hypersensitivity of DNA polymerase β knockout mouse embryonic fibroblasts
Apurinic/apyrimidinic (AP) endonuclease (Apex) is required for base excision repair (BER), which is the major mechanism of repair for small DNA lesions such as alkylated bases. Apex incises the DNA strand at an AP site to leave 3'-OH and 5'-deoxyribose phosphate (5'-dRp) termini. DNA polymerase β (PolB) plays a dominant role in single nucleotide (Sn-) BER by incorporating a nucleotide and remov...
متن کاملHuman DNA polymerase iota: wrong for the right reasons.
In a recent collaborative study, Thomas A. Kunkel, director of the NIEHS Environmental Biology Program, NIEHS deputy director Samuel H. Wilson, and Roger Woodgate, an investigator at the National Institute of Child Health and Human Development, report novel findings about DNA repair and genome stability in humans. Woodgate's laboratory recently discovered and purified polymerase iota (pol ι), o...
متن کاملDNA repair synthesis in human fibroblasts requires DNA polymerase delta.
When UV-irradiated cultured diploid human fibroblasts were permeabilized with Brij-58 then separated from soluble material by centrifugation, conservative DNA repair synthesis could be restored by a soluble factor obtained from the supernatant of similarly treated HeLa cells. Extensive purification of this factor yielded a 10.2 S, 220,000-dalton polypeptide with the DNA polymerase and 3'- to 5'...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: DNA Repair
سال: 2007
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2006.08.005